6WPM

Crystal structure of a putative oligosaccharide periplasmic-binding protein from Synechococcus sp. MITs9220 in complex with zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 43 2 2
Unit cell lengths	144.778, 144.778, 53.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.780 - 2.880
R-factor	0.2102
Rwork	0.208
R-free	0.25390
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.494
Data reduction software	pointless
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.420	2.980
High resolution limit [Å]	2.230	2.880
Rmerge	0.072	0.027
Rmeas		0.038
Rpim		0.027
Number of reflections	13405	1253
<I/σ(I)>	0.0694	30
Completeness [%]	99.4	94.6
Redundancy	3.8	2
CC(1/2)		0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	Ammonium sulphate (2 M), PEG 3350 (20% w/v)