6WPI

Crystal structure of Nop9 in complex with ITS1 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	71.925, 119.540, 142.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.750 - 3.020
R-factor	0.1984
Rwork	0.195
R-free	0.23220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5svd
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.069	0.037	0.967
Rmeas	0.070	0.039
Rpim	0.020	0.012	0.258
Total number of observations	354389
Number of reflections	24604	1328	1185
<I/σ(I)>	8.2
Completeness [%]	99.8	97.1	100
Redundancy	14.4	10.7	15
CC(1/2)		0.999	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.75	293	18% (w/v) PEG 3350, 0.2 M ammonium citrate, 0.1 M MES