6WPC
Crystal structure of Bacillus thuringiensis Cry1A.2 tryptic core variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 120.578, 225.820, 239.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.120 - 2.990 |
| R-factor | 0.2008 |
| Rwork | 0.198 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4arx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.556 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.000 | 3.050 |
| High resolution limit [Å] | 2.990 | 3.000 |
| Rmerge | 0.182 | 0.483 |
| Number of reflections | 62795 | 1992 |
| <I/σ(I)> | 7.4 | 1.33 |
| Completeness [%] | 95.1 | 60.8 |
| Redundancy | 3.47 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Wizard 12 screen H7 (10% PEG8000, 0.1 M Tris, pH 7.0, 0.2 M magnesium chloride) |
