6WP8
Proton-pumping mutant of Mastigocladopsis repens rhodopsin chloride pump
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 103.741, 103.741, 55.213 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.920 - 2.500 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6NWF |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.661 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.920 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 11125 | 1275 |
| <I/σ(I)> | 3.3 | 1 |
| Completeness [%] | 92.0 | 94.6 |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.980 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 307.15 | 3.6 M sodium phosphate monobasic monohydrate, pH 4.0, 180 mM 1,6 hexanediol, 3.5% triethylene glycol |
