6WOR

Structure of the broadly neutralizing antibody HC1AM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	65.639, 80.966, 181.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.518 - 2.601
R-factor	0.2207
Rwork	0.219
R-free	0.25290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6woq
RMSD bond length	0.002
RMSD bond angle	0.603
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	15091	640
<I/σ(I)>	26.1
Completeness [%]	99.1
Redundancy	7.4
CC(1/2)	0.940	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	17% (w/v) PEG 4000, 15% (v/v) glycerol, 8.5% (v/v) isopropanol, 0.085M Na-HEPES pH 7.5