6WO5

Structure of Hepatitis C Virus Envelope Glycoprotein E2 core from genotype 1a bound to neutralizing antibody 212.1.1 and non neutralizing antibody E1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	55.793, 242.204, 100.729
Unit cell angles	90.00, 98.31, 90.00

Refinement procedure

Resolution	47.435 - 2.619
R-factor	0.2051
Rwork	0.203
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mwf
RMSD bond length	0.004
RMSD bond angle	0.799
Data reduction software	HKL-2000 (3.25)
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.670
High resolution limit [Å]	2.619	2.619
Number of reflections	76536	2986
<I/σ(I)>	16.3
Completeness [%]	96.2
Redundancy	4.8
CC(1/2)	0.960	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	from 20% (w/v) PEG 3000, 0.2M NaCl, 0.1M HEPES pH 7.5