6WMI

ZNF410 zinc fingers 1-5 with 17 mer blunt DNA Oligonucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 62
Unit cell lengths	186.257, 186.257, 46.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.920 - 2.750
R-factor	0.1609
Rwork	0.159
R-free	0.19410
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.683
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.920	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.200	0.500
Rpim	0.070	0.820
Number of reflections	47241	4697
<I/σ(I)>	12.3	1.3
Completeness [%]	99.9	100
Redundancy	11.1	10
CC(1/2)	0.991	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M ammonium formate, 20% PEG3350