6WMC
Crystal structure of a soluble variant of full-length human APOBEC3G (pH 9.0)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-08 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 60.233, 42.091, 191.911 |
| Unit cell angles | 90.00, 95.94, 90.00 |
Refinement procedure
| Resolution | 47.720 - 3.490 |
| R-factor | 0.2222 |
| Rwork | 0.216 |
| R-free | 0.27420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6BUX CHAIN A AND 5K81 CHAIN A |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 0.158 | 0.497 |
| Number of reflections | 5588 | 482 |
| <I/σ(I)> | 5.08 | 1.37 |
| Completeness [%] | 89.0 | 83.4 |
| Redundancy | 2.8 | 2.6 |
| CC(1/2) | 0.985 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 0.1M BICINE (pH 9.0) and 10% w/v PEG 6000 |
