6WMA
Crystal structure of a soluble variant of full-length human APOBEC3G (pH 7.6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 59.940, 41.870, 191.541 |
| Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
| Resolution | 31.717 - 2.500 |
| R-factor | 0.1993 |
| Rwork | 0.197 |
| R-free | 0.24760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6BUX CHAIN A AND 5K81 CHAIN A |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.720 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.107 | 0.423 |
| Number of reflections | 14930 | 1301 |
| <I/σ(I)> | 8.1 | 2.7 |
| Completeness [%] | 90.1 | 80.8 |
| Redundancy | 3 | 2.8 |
| CC(1/2) | 0.990 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 277 | 0.03 M Citric acid, 0.07 M BIS-TRIS propane (pH 7.6) and 20% w/v PEG 3350 |
