6WLG
Ints3 C-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 |
| Unit cell lengths | 234.854, 234.854, 47.567 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.317 - 3.111 |
| R-factor | 0.2065 |
| Rwork | 0.204 |
| R-free | 0.26330 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.777 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.320 | 3.330 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Rmerge | 0.060 | 0.570 |
| Number of reflections | 17495 | 3181 |
| <I/σ(I)> | 14.6 | 2.5 |
| Completeness [%] | 99.5 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.998 | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Magnesium Acetate, 13% PEG 3350 |
