6WKP

Crystal structure of RNA-binding domain of nucleocapsid phosphoprotein from SARS CoV-2, monoclinic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-07
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	33.388, 71.848, 106.961
Unit cell angles	90.00, 92.98, 90.00

Refinement procedure

Resolution	35.610 - 2.670
R-factor	0.2001
Rwork	0.197
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vyo
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.670	7.320	2.700
Rmerge	0.091	0.043	0.597
Rmeas	0.102	0.048	0.684
Rpim	0.046	0.021	0.326
Number of reflections	14108	746	665
<I/σ(I)>	8.2
Completeness [%]	97.5	98.3	90
Redundancy	4.5	4.7	3.8
CC(1/2)		0.994	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.1 M MES, 30% PEG4000