6WK5

Crystal structure of Gdx-Clo from Small Multidrug Resistance family of transporters

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	80
Detector technology	CCD
Collection date	2019-10-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	C 1 2 1
Unit cell lengths	141.800, 51.093, 108.433
Unit cell angles	90.00, 93.08, 90.00

Refinement procedure

Resolution	44.230 - 3.500
R-factor	0.2534
Rwork	0.252
R-free	0.28580
Structure solution method	SAD
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.140	3.830
High resolution limit [Å]	3.500	3.500
Rmerge	0.060	0.148
Rmeas	0.070	0.171
Rpim	0.035	0.086
Number of reflections	10080	2389
<I/σ(I)>	17.3
Completeness [%]	100.0
Redundancy	7.5
CC(1/2)	0.995	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	294	0.1 M LiNO3, 0.1 M N-(2-Acetamido)iminodiacetic acid (ADA) pH 6.8, and 35% PEG 600