6WJH

Crystal structure of MAGE-A11 bound to the PCF11 degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-01
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	55.493, 78.195, 72.911
Unit cell angles	77.44, 77.34, 89.59

Refinement procedure

Resolution	38.790 - 2.190
R-factor	0.2069
Rwork	0.205
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v0p
RMSD bond length	0.008
RMSD bond angle	1.480
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.790	2.250
High resolution limit [Å]	2.190	2.190
Number of reflections	56569	2745
<I/σ(I)>	19.9
Completeness [%]	96.6
Redundancy	2.4
CC(1/2)	0.952	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	The well solution contained 0.2 M Na acetate, 0.1 M Bis Tris propane pH 7.5 and 20% (w/v) PEG 3350. The drop contained a 1:1 volume ratio of well solution to protein (15 mg/ml in 50 mM Tris, pH 7.4, 200 mM NaCl and 5 mM DTT).