6WJE

Copper resistance protein copG- Form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	63.700, 87.460, 143.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.420 - 2.500
R-factor	0.1938
Rwork	0.188
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wis
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3724)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.420	2.570
High resolution limit [Å]	2.500	2.500
Rmeas	0.148
Number of reflections	28435	2067
<I/σ(I)>	8.93	1.9
Completeness [%]	99.8
Redundancy	6.62
CC(1/2)	0.996	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	100 mM Na Cacodylate pH 6.8 200 mM Zn Acetate 19% ethanol