6WIG

Structure of STENOFOLIA Protein HD domain bound with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-03
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97935
Spacegroup name	P 1 21 1
Unit cell lengths	48.061, 49.489, 69.810
Unit cell angles	90.00, 106.46, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.1997
Rwork	0.194
R-free	0.24980
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.214
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.080	0.662
Number of reflections	17541	1431
<I/σ(I)>	4.4
Completeness [%]	95.2	77.7
Redundancy	8.1
CC(1/2)		0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	.15 M sodium chloride 28 % v/v PEG Smear Medium