6WI7

RING1B-BMI1 fusion in closed conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	49.311, 54.353, 82.266
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.521 - 1.702
R-factor	0.1662
Rwork	0.165
R-free	0.19570
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	0.943
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	24900	24900
<I/σ(I)>	24.18
Completeness [%]	100.0
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277.15	100 mM HEPES, pH 7.5, 20% PEG8000