6WHB
MEKK1 TOG domain (548-867)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-01 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 71.817, 71.817, 259.561 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.291 - 1.900 |
R-factor | 0.208269068866 |
Rwork | 0.207 |
R-free | 0.22969 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 0.728 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.145 | |
Rmeas | 0.147 | |
Number of reflections | 27403 | 2683 |
<I/σ(I)> | 19.36 | |
Completeness [%] | 99.0 | 100 |
Redundancy | 28.6 | 29 |
CC(1/2) | 1.000 | 0.239 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 290.15 | 0.1 M Bis-Tris Propane pH 6.9, 1.5 M sodium acetate |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 290.15 | 0.1 M Bis-Tris Propane pH 6.9, 1.5 M sodium acetate |