6WG6
Crystal structure of human SMC1-SMC3 hinge domain heterodimer in north-open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 123.985, 145.021, 315.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.500 - 3.540 |
| R-factor | 0.253 |
| Rwork | 0.252 |
| R-free | 0.28830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wd5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.742 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.610 |
| High resolution limit [Å] | 3.540 | 3.540 |
| Number of reflections | 61430 | 2413 |
| <I/σ(I)> | 5 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 13 |
| CC(1/2) | 0.974 | 0.177 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293.15 | 0.1 M sodium acetate pH 4.0, 15% polyethylene glycol 400 |
