6WG6

Crystal structure of human SMC1-SMC3 hinge domain heterodimer in north-open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	123.985, 145.021, 315.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.500 - 3.540
R-factor	0.253
Rwork	0.252
R-free	0.28830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wd5
RMSD bond length	0.004
RMSD bond angle	0.742
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.610
High resolution limit [Å]	3.540	3.540
Number of reflections	61430	2413
<I/σ(I)>	5	1
Completeness [%]	100.0	100
Redundancy	12.8	13
CC(1/2)	0.974	0.177

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293.15	0.1 M sodium acetate pH 4.0, 15% polyethylene glycol 400