6WF0
Crystal Structure of Broadly Neutralizing Antibody 3I14 Bound to the Influenza A H3 Hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2017-04-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | .97919 |
| Spacegroup name | P 63 |
| Unit cell lengths | 130.318, 130.318, 188.552 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.660 - 3.460 |
| R-factor | 0.2353 |
| Rwork | 0.231 |
| R-free | 0.28190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 405I |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.652 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.660 | 3.581 |
| High resolution limit [Å] | 3.457 | 3.457 |
| Rmerge | 0.196 | 0.992 |
| Rmeas | 0.216 | 1.095 |
| Rpim | 0.090 | 0.459 |
| Number of reflections | 23612 | 2349 |
| <I/σ(I)> | 8.92 | 1.74 |
| Completeness [%] | 99.1 | 98.7 |
| Redundancy | 5.5 | |
| CC(1/2) | 0.989 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18-24% w/v PEG 3350, 0.2M KCN, and 0.1M Bis-Tris-Propane pH7.5 |
