6WE9

YTH domain of human YTHDC1 with 11mer ssDNA Containing N6mA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	119.966, 42.535, 90.019
Unit cell angles	90.00, 103.46, 90.00

Refinement procedure

Resolution	35.310 - 1.590
R-factor	0.2231
Rwork	0.222
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.003
RMSD bond angle	0.555
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.310	1.650
High resolution limit [Å]	1.590	1.590
Rmerge	0.204	0.693
Rpim	0.094	0.543
Number of reflections	54367	3725
<I/σ(I)>	10.4	1.9
Completeness [%]	91.6
Redundancy	4.7	1.7
CC(1/2)		0.468

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292	0.1 M Bis-Tris pH 6.0, 0.2 M ammonium sulfate, 29% (w/v) polyethylene glycol (PEG) 3350