6WE8

YTH domain of human YTHDC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	39.914, 103.960, 42.201
Unit cell angles	90.00, 104.87, 90.00

Refinement procedure

Resolution	20.010 - 1.180
R-factor	0.1664
Rwork	0.166
R-free	0.19470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.002
RMSD bond angle	0.578
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.010	1.220
High resolution limit [Å]	1.180	1.180
Rmerge	0.072	0.579
Rpim	0.038	0.517
Number of reflections	93137	2859
<I/σ(I)>	22.2	1.2
Completeness [%]	86.1	26.5
Redundancy	5.8	1.4
CC(1/2)		0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	292	0.1-0.15M ammonium sulfate 0.1 M Bis-Tris pH 5.5 25-29% PEG 3350