6WE0

Wheat dwarf virus Rep domain complexed with a single-stranded DNA 10-mer comprising the cleavage site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	45.572, 50.010, 73.441
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.340 - 1.800
R-factor	0.193
Rwork	0.192
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6q1m
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.340	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.075	0.851
Rmeas	0.084	0.963
Number of reflections	15366	1551
<I/σ(I)>	14.17	2.05
Completeness [%]	95.2	98.54
Redundancy	4.4	4.3
CC(1/2)	0.997	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	12% w/v PEG 8000, 0.1 M sodium cocadylate, pH 6.5, 0.2M zinc acetate, 25% glycerol