6WDP

Interleukin 12 receptor subunit beta-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	55.780, 55.780, 182.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.190 - 2.010
R-factor	0.223
Rwork	0.222
R-free	0.24170
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	0.720
Data reduction software	XDS
Data scaling software	XDS
Phasing software	autoSHARP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.190	2.600	2.082
High resolution limit [Å]	2.010	2.500	2.010
Rmerge	0.103	0.840	1.685
Rmeas	0.108	0.908	1.789
Rpim	0.032	0.341	0.581
Number of reflections	19869	1159	1861
<I/σ(I)>	13.8	3.1	1.16
Completeness [%]	98.8	99.4	94.03
Redundancy	11	12.8	9
CC(1/2)	0.999	0.824	0.438

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.9	293	1.75 M ammonium sulfate, 0.2 M NaCl, and 0.1 M sodium cacodylate pH 5.9
2
2
2