6WCY

N160D Deamidation Mutant of Human gammaD-Crystallin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-05
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	50.811, 51.872, 113.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.590 - 1.204
R-factor	0.1701
Rwork	0.169
R-free	0.19440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hk0
RMSD bond length	0.009
RMSD bond angle	1.208
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.590	1.247
High resolution limit [Å]	1.204	1.204
Rmerge	0.053	1.185
Rmeas	0.055
Rpim	0.015
Number of reflections	85435	4861
<I/σ(I)>	22.5	1
Completeness [%]	90.9	52.45
Redundancy	12.9
CC(1/2)	0.999	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M succinic acid (pH 7.0), 15% w/v PEG 3350