6WCU

Crystal structure of coiled coil region of human septin 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	22.593, 26.114, 45.468
Unit cell angles	90.00, 104.28, 90.00

Refinement procedure

Resolution	22.465 - 1.800
R-factor	0.2267
Rwork	0.219
R-free	0.24320
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	Arcimboldo
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.110	26.110	1.840
High resolution limit [Å]	1.800	8.630	1.800
Rmerge	0.060	0.050	0.213
Rmeas	0.073	0.062	0.259
Rpim	0.040	0.035	0.145
Total number of observations	14437	105	950
Number of reflections	4769	40	306
<I/σ(I)>	11.3	16.7	4.5
Completeness [%]	97.4	82.9	97.1
Redundancy	3	2.6	3.1
CC(1/2)	0.995	0.997	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M citric acid pH 5.0, 1.6 M ammonium sulfate