6WC6
Crystal structure of a truncated LSD1:CoREST in the presence of an LSD1-NT peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-08-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 121.783, 179.164, 234.907 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.720 - 3.100 |
R-factor | 0.2171 |
Rwork | 0.216 |
R-free | 0.23580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xbf |
RMSD bond length | 0.003 |
RMSD bond angle | 0.579 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Refinement software | REFMAC (5-3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 142.460 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 46959 | 4642 |
<I/σ(I)> | 13.7 | 1.6 |
Completeness [%] | 99.9 | |
Redundancy | 15 | |
CC(1/2) | 0.998 | 0.764 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | The LSD1-CoREST complex 10mg/ml concentration (in 25 mM HEPES-Na, pH 7.4, 100 mM NaCl, 1 mM TCEP) was mixed (1:1) with the reservoir solution [0.60 M Li2SO4, 0.63 M (NH4)2SO4, 0.25 M NaCl, 100 mM Na-citrate, pH 5.6, 5 mM TCEP]. When get crystals, soak the crystals within 5mM LSD1-NT peptide. |