6WBR

Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNCC-3' PAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	82.313, 119.348, 177.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.650 - 2.910
R-factor	0.2262
Rwork	0.224
R-free	0.26610
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.128
Data reduction software	XDS
Data scaling software	AutoProcess
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	142.600	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.476	2.000
Rmeas	0.486
Rpim	0.099	0.781
Number of reflections	55715	4272
<I/σ(I)>	6.1	0.9
Completeness [%]	98.3	97.5
Redundancy	7.2	7.4
CC(1/2)	0.976	0.362

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		303.15	0.04 M Citric acid, 0.06 M Bis-tris propane and 15-20% polyethylene glycol 3350