6WBP

Crystal structure of coiled coil region of human septin 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9200
Spacegroup name	P 61 2 2
Unit cell lengths	49.640, 49.640, 228.549
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.990 - 1.800
R-factor	0.2174
Rwork	0.216
R-free	0.25470
Structure solution method	AB INITIO PHASING
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	Arcimboldo
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.140	57.140	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.065	0.034	1.147
Rmeas	0.067	0.036	1.204
Rpim	0.017	0.010	0.361
Total number of observations	242344	2295	9957
Number of reflections	16541	205	909
<I/σ(I)>	19.5	47.5	2
Completeness [%]	99.8	99.9	97.6
Redundancy	14.7	11.2	11
CC(1/2)	1.000	0.999	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.3	291	0.1 M citric acid pH 4.3, 1.8 M ammonium sulfate