6WBH
Crystal structure of mRECK(CC4) in fusion with engineered MBP at medium resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-10 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.222, 110.599, 57.791 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.530 - 2.455 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3set |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.8.1) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.530 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.190 | 0.511 |
| Rmeas | 0.208 | 0.562 |
| Rpim | 0.081 | 0.222 |
| Number of reflections | 19054 | 1811 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 98.3 | 94.52 |
| Redundancy | 6.3 | 5.7 |
| CC(1/2) | 0.947 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 293 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, 25% PEG 4000 |
