6WBA

Structure of Mouse Importin alpha MLH1- R470A NLS Peptide Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-20
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	1.425
Spacegroup name	P 21 21 21
Unit cell lengths	78.389, 90.354, 99.333
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.524 - 2.151
R-factor	0.1731
Rwork	0.172
R-free	0.19730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u5p
RMSD bond length	0.004
RMSD bond angle	0.685
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.524	2.228
High resolution limit [Å]	2.150	2.151
Rmerge	0.099	1.327
Rmeas	0.102	1.380
Rpim	0.028	0.373
Number of reflections	508565	3719
<I/σ(I)>	23.22	2
Completeness [%]	99.0
Redundancy	13.2
CC(1/2)	0.990	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1 M sodium citrate (pH 6), 0.6 M sodium citrate and 10 mM DTT