6WB7
Acarbose Kinase AcbK as a Complex with Acarbose and AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.545, 50.268, 127.794 |
| Unit cell angles | 89.06, 80.18, 71.81 |
Refinement procedure
| Resolution | 28.532 - 2.440 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wb4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.790 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.533 | 28.530 | 2.500 |
| High resolution limit [Å] | 2.440 | 10.910 | 2.440 |
| Rmerge | 0.136 | 0.040 | 0.665 |
| Rmeas | 0.173 | 0.052 | 0.852 |
| Rpim | 0.106 | 0.032 | 0.525 |
| Total number of observations | 1139 | 7020 | |
| Number of reflections | 40165 | 438 | 2895 |
| <I/σ(I)> | 5.6 | 18.4 | 1.4 |
| Completeness [%] | 97.3 | 93.4 | 92.4 |
| Redundancy | 2.4 | 2.6 | 2.4 |
| CC(1/2) | 0.983 | 0.996 | 0.562 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.25 M NaCl, 16% w/v PEG 3350, 10 mM MnCl2, 6 mM AMP-PNP, and 50 mM Acarbose |
