6WB5
Microbiome-derived Acarbose Kinase Mak1 as a Complex with Acarbose and AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-13 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 65 |
| Unit cell lengths | 86.551, 86.551, 164.334 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.330 - 3.102 |
| R-factor | 0.1679 |
| Rwork | 0.163 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wb4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.330 | 28.330 | 3.180 |
| High resolution limit [Å] | 3.100 | 13.870 | 3.100 |
| Rmerge | 0.171 | 0.041 | 1.085 |
| Rmeas | 0.177 | 0.042 | 1.126 |
| Rpim | 0.046 | 0.012 | 0.295 |
| Total number of observations | 181665 | 1945 | 12569 |
| Number of reflections | 12632 | 140 | 893 |
| <I/σ(I)> | 16 | 51.6 | 2.7 |
| Completeness [%] | 99.7 | 89.8 | 97.4 |
| Redundancy | 14.4 | 13.9 | 14.1 |
| CC(1/2) | 0.998 | 0.999 | 0.821 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.25 M Ammonium Dihydrogen Phosphate, 0.1M Tris-HCl pH 8.3, 10 mM MnCl2, 6 mM AMP-PNP, 50 mM Acarbose |
