6WAY
C-terminal SH2 domain of p120RasGAP in complex with p190RhoGAP phosphotyrosine peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.119, 83.910, 54.239 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.278 - 1.500 |
| R-factor | 0.1658 |
| Rwork | 0.165 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gsb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.809 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.149 | 0.093 | 0.887 |
| Rmeas | 0.152 | 0.095 | 0.938 |
| Rpim | 0.031 | 0.019 | 0.293 |
| Total number of observations | 492046 | ||
| Number of reflections | 23209 | 2457 | 2266 |
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 100.0 | 100 | 99.8 |
| Redundancy | 21.2 | 24.8 | 8.6 |
| CC(1/2) | 0.998 | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.2 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5 |
