6WAS

Structure of D19.PA8 Fab in complex with 1FD6 16055 V1V2 scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-01
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	73.697, 97.893, 168.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.903 - 1.904
R-factor	0.19
Rwork	0.189
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tdn
RMSD bond length	0.004
RMSD bond angle	0.856
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	84549	1342
<I/σ(I)>	18.4
Completeness [%]	99.2
Redundancy	7.1
CC(1/2)	0.991	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	0.1 M Tris, pH 8.5, 18% PEG6000