6WAC
FF248-249AA PCNA mutant defective in BIR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-04-15 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 86.881, 86.881, 100.724 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.280 - 2.900 |
| R-factor | 0.2383 |
| Rwork | 0.237 |
| R-free | 0.27010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1plq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.532 |
| Data reduction software | TRUNCATE |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | Coot (0.8.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.280 | 3.003 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.079 | 0.837 |
| Rpim | 0.057 | 0.604 |
| Number of reflections | 12383 | 595 |
| <I/σ(I)> | 13.4 | 2 |
| Completeness [%] | 99.6 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | magnesium chloride hexahydrate, sodium dimethylarsinic acid, PEG1000 |
