6W9Z

De novo designed receptor transmembrane domains enhance CAR-T cytotoxicity and attenuate cytokine release

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95366
Spacegroup name	P 2 21 21
Unit cell lengths	44.100, 56.205, 91.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.738 - 2.700
R-factor	0.2244
Rwork	0.219
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eh6
Data reduction software	XDS (Jan 26, 2018 BUILT=20180319)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.740	2.796
High resolution limit [Å]	2.700	2.700
Rmerge	0.070	0.855
Rmeas	0.080	0.969
Rpim	0.037	0.449
Number of reflections	6655	444
<I/σ(I)>	13.26	1.86
Completeness [%]	96.1	68.62
Redundancy	4.4	4.4
CC(1/2)	0.999	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.7	293	40 mg/ml peptide in LCP 8 v/v 2-methyl-2,4-pentanediol 0.1 M ADA pH 6.7 0.4 M potassium nitrate 0.1 M tripotassium citrate