6W9P
Tel26 Parallel Four-quartet G-quadruplex with K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 196 |
| Detector technology | CCD |
| Collection date | 2020-02-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 38.681, 38.681, 64.921 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.500 - 1.990 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o0k |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.722 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.900 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.099 | 0.875 |
| Number of reflections | 4116 | |
| <I/σ(I)> | 11.1 | 1.6 |
| Completeness [%] | 99.1 | 94.6 |
| Redundancy | 5.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 32% PEG 2000, 0.3M sodium chloride, 0.05M sodium cacodylate pH 6.5 |
