6W89

Structure of DNMT3A (R882H) in complex with CGA DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	187.559, 187.559, 82.398
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.720 - 2.499
R-factor	0.1968
Rwork	0.196
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yx2
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.720	2.590
High resolution limit [Å]	2.499	2.500
Rmerge	0.078
Number of reflections	110518	110518
<I/σ(I)>	10.4
Completeness [%]	98.5
Redundancy	6.7
CC(1/2)	0.999	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Sodium Citrate pH 4.2, 1% PEG8000