6W83
K2P2.1 (TREK-1), 100 mM K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.222, 121.164, 129.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.950 - 3.901 |
| R-factor | 0.2458 |
| Rwork | 0.242 |
| R-free | 0.31160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cq6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.590 | 4.360 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.259 | |
| Number of reflections | 10566 | 2932 |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11.7 | |
| CC(1/2) | 0.113 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 22-25% PEG400, 100mM HEPES pH=8.0, 1mM CdCl2, 200mM KCl |
