6W7Z

RNF12 RING domain in complex with Ube2d2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	P 1 21 1
Unit cell lengths	48.441, 50.453, 54.375
Unit cell angles	90.00, 99.72, 90.00

Refinement procedure

Resolution	47.740 - 1.800
R-factor	0.1795
Rwork	0.178
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SAD-phased structure of RNF12-Ube2d2 (undeposited model)
RMSD bond length	0.002
RMSD bond angle	0.530
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.740	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	0.711
Rmeas	0.080	0.817
Rpim	0.039	0.397
Total number of observations	99121
Number of reflections	24099	1405
<I/σ(I)>	12.3
Completeness [%]	99.9	99.9
Redundancy	4.1	4.1
CC(1/2)	0.998	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	15-20% PEG 3350, 100-200 mM ammonium formate