6W7V

Structure of rabbit actin in complex with truncated analog of Mycalolide B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	37.763, 74.869, 61.197
Unit cell angles	90.00, 93.02, 90.00

Refinement procedure

Resolution	37.710 - 1.700
R-factor	0.1808
Rwork	0.179
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mgo
RMSD bond length	0.014
RMSD bond angle	1.439
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.710	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	43140	3601
<I/σ(I)>	12.4
Completeness [%]	97.9	97.38
Redundancy	6.5
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1M Tris-HCl, pH 8.5, 25% PEG3350