6W7P

Crystal Structure Analysis of Space-grown Lysozyme - Ground experiment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.19499
Spacegroup name	P 43 21 2
Unit cell lengths	78.866, 78.866, 37.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.600
R-factor	0.1727
Rwork	0.170
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kxk
RMSD bond length	0.020
RMSD bond angle	1.943
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	EPMR
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.767	39.433	1.690
High resolution limit [Å]	1.600	5.060	1.600
Rmerge		0.027	0.619
Rmeas	0.054	0.032	0.755
Rpim	0.028	0.016	0.426
Total number of observations		1752	6888
Number of reflections	15331	485	2288
<I/σ(I)>	10.9	30.1	1.7
Completeness [%]	96.2	82.8	99.6
Redundancy	3.2	3.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	4.6	296	PEG 6000, NaCl, Na Acetate