6W5Y

Inferred receptor binding domain of human endogenous retrovirus envelope EnvP(b)1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9998
Spacegroup name	C 1 2 1
Unit cell lengths	243.310, 37.350, 66.990
Unit cell angles	90.00, 102.28, 90.00

Refinement procedure

Resolution	49.561 - 2.500
R-factor	0.2237
Rwork	0.222
R-free	0.25380
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.988
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.561	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.127	0.886
Rmeas	0.142	0.993
Rpim	0.063	0.442
Number of reflections	20893	2023
<I/σ(I)>	12.65	1.83
Completeness [%]	99.8	99.75
Redundancy	4.9	5
CC(1/2)	0.996	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	0.5 M Sodium chloride, 0.1 M Bis- tris(hydroxymethyl)aminomethane propane pH 7.0, 20 %(w/v) polyethylene glycol 4000