6W5Y
Inferred receptor binding domain of human endogenous retrovirus envelope EnvP(b)1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 243.310, 37.350, 66.990 |
| Unit cell angles | 90.00, 102.28, 90.00 |
Refinement procedure
| Resolution | 49.561 - 2.500 |
| R-factor | 0.2237 |
| Rwork | 0.222 |
| R-free | 0.25380 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.988 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.561 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.127 | 0.886 |
| Rmeas | 0.142 | 0.993 |
| Rpim | 0.063 | 0.442 |
| Number of reflections | 20893 | 2023 |
| <I/σ(I)> | 12.65 | 1.83 |
| Completeness [%] | 99.8 | 99.75 |
| Redundancy | 4.9 | 5 |
| CC(1/2) | 0.996 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 0.5 M Sodium chloride, 0.1 M Bis- tris(hydroxymethyl)aminomethane propane pH 7.0, 20 %(w/v) polyethylene glycol 4000 |
