6W5B

N124D Deamidation Mutant of Human gammaD-Crystallin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.8856
Spacegroup name	P 21 21 21
Unit cell lengths	33.649, 51.493, 93.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.170 - 1.150
R-factor	0.152
Rwork	0.151
R-free	0.17220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hk0
RMSD bond length	0.011
RMSD bond angle	1.214
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.170	1.191
High resolution limit [Å]	1.150	1.150
Rmerge	0.068	0.519
Rmeas	0.071	0.575
Rpim	0.020	0.241
Number of reflections	58473	5481
<I/σ(I)>	17.43	2.2
Completeness [%]	99.3	93.9
Redundancy	11.6	5.5
CC(1/2)	0.999	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M ammonium chloride, 20% w/v PEG 3350