6W52
Prefusion RSV F bound by neutralizing antibody RSB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 190.298, 190.298, 190.298 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.570 - 3.740 |
| R-factor | 0.2251 |
| Rwork | 0.218 |
| R-free | 0.28630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mmu |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.800 |
| High resolution limit [Å] | 3.740 | 3.740 |
| Rmerge | 0.096 | |
| Number of reflections | 11995 | 1188 |
| <I/σ(I)> | 20.79 | |
| Completeness [%] | 99.8 | |
| Redundancy | 11.2 | |
| CC(1/2) | 0.998 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M Hepes pH 7.5, 25% PEG 2000 MME, 0.01 M spermidine |
