6W4V
Structure of anti-ferroportin Fab45D8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 170 |
| Detector technology | PIXEL |
| Collection date | 2019-10-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.097, 36.907, 86.514 |
| Unit cell angles | 90.00, 112.99, 90.00 |
Refinement procedure
| Resolution | 39.820 - 2.100 |
| R-factor | 0.2168 |
| Rwork | 0.216 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bzv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.918 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.820 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 24736 | 1832 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.996 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.3 M trimethylamine-N-oxide (TMAO), 0.1 M Tris, pH 8.5, 30% w/v PEG2000 MME |
