6W3L
APE1 exonuclease substrate complex wild-type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-20 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.371, 65.196, 91.258 |
| Unit cell angles | 90.00, 110.27, 90.00 |
Refinement procedure
| Resolution | 24.770 - 2.590 |
| R-factor | 0.2221 |
| Rwork | 0.217 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wn4 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.502 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.770 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.600 |
| Rmeas | 0.123 | 1.000 |
| Number of reflections | 44162 | 2112 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 7% PEG20000, 100 mM sodium citrate, 15% glycerol, 5 mM calcium chloride |
