6W38

Crystal structure of the FAM46C/Plk4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	I 2 2 2
Unit cell lengths	87.392, 158.613, 165.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.649 - 4.480
R-factor	0.2381
Rwork	0.232
R-free	0.29550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n7z
RMSD bond length	0.004
RMSD bond angle	1.185
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.580
High resolution limit [Å]	4.480	4.500
Rpim	0.025
Number of reflections	6713	271
<I/σ(I)>	22.2
Completeness [%]	94.0
Redundancy	6.2
CC(1/2)		0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	10	293	0.2 M Tris, pH 10