6W1F
Crystal structure of Streptococcus thermophilus SHP pheromone receptor Rgg3 bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 |
Unit cell lengths | 90.790, 90.790, 245.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.395 - 3.200 |
R-factor | 0.2217 |
Rwork | 0.217 |
R-free | 0.26620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yv6 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
Rmerge | 0.054 | 0.031 | 0.664 |
Rmeas | 0.057 | 0.032 | 0.699 |
Rpim | 0.018 | 0.010 | 0.216 |
Number of reflections | 18799 | 968 | 928 |
<I/σ(I)> | 8 | ||
Completeness [%] | 99.5 | 98.9 | 99.9 |
Redundancy | 10.3 | 10.2 | 10.3 |
CC(1/2) | 0.986 | 0.997 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 298 | 170 mM ammonium acetate, 85 mM sodium citrate, 22% PEG 4,000, and 15% glycerol |