6W1D

Structure of human mitochondrial complex Nfs1-ISCU2 (WT)-ISD11 with E.coli ACP1 at 1.8 A resolution (NIAU)2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-17
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	86.348, 86.348, 245.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.927 - 1.795
R-factor	0.1595
Rwork	0.159
R-free	0.18860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wlw
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.927	1.859
High resolution limit [Å]	1.795	1.795
Number of reflections	87545	8566
<I/σ(I)>	12.19
Completeness [%]	99.9	99.73
Redundancy	14.5	14
CC(1/2)	0.997	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	288	0.1 M MES pH 6.0-6.5 22 % PEG 400